6533b7d8fe1ef96bd1269af9
RESEARCH PRODUCT
Development and validation of a micellar liquid chromatographic method to determine three antitumorals in plasma
Enrique Ochoa-arandaJuan Peris-vicenteJaume Albiol ChivaJosep Esteve Romerosubject
Clinical BiochemistryAntineoplastic Agents01 natural sciencesMicelleAnalytical ChemistryAbsorbance03 medical and health sciences0302 clinical medicineLimit of DetectionNeoplasmsCalibrationHumansGeneral Pharmacology Toxicology and PharmaceuticsMicellesDetection limitAqueous solutionChromatographyPlasma samplesChemistry010401 analytical chemistryGeneral MedicinePlasmaFactorial experiment0104 chemical sciencesMedical Laboratory TechnologyCase-Control Studies030220 oncology & carcinogenesisBlood Chemical AnalysisChromatography Liquiddescription
Aim: A micellar liquid chromatographic method to determine several anticancer drugs (pazopanib, dabrafenib and regorafenib) in plasma was developed and validated by the guidelines of the EMA. Experimental: Plasma samples were directly injected, after a 1/5-dilution in a micellar solution. The drugs were resolved in <18 min using a C18 column. The mobile phase was an aqueous solution of 0.12 M SDS – 2% 1-pentanol, buffered at pH 7. The detection was performed by absorbance at 260 nm. Results: The values of the main validation parameters were: LOD (0.1–1 mg/l), calibration range (0.2–2 to 80 mg/l), accuracy (-12.5 to +11.7%) and precision (<11.9%). Conclusion: The procedure was conducted by minimum cost, effort, manipulation, time and quantity of hazardous chemicals. The method was useful to determine the drugs at their respective target concentrations, and was found useful for clinical analysis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-05-19 | Bioanalysis |