6533b7d8fe1ef96bd1269b64
RESEARCH PRODUCT
An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples
Wojciech ŁUczajGiuseppe PoliMartina PodborskaCinzia MasciaNicolle BreusingCorinne M. SpickettNorsyahida Mohd FauziIngrid WiswedelTilman GruneMaria Cristina MunteanuAnca DinischiotuRosario CasillasDaniela GradinaruSieglinde ZelzerAntonín LojekLaura BravoSuzana BorovićMaria Teresa MitjavilaNeven ZarkovicFiorella BiasiJuan GambiniHeidemarie FaberLidija MrakovcicGrzegorz BartoszIsidre CasalsRaquel MateosLuka AndrisicElżbieta SkrzydlewskaJose ViñaAndreas MeinitzerPaulina SicinskaJoanna DrzewinskaMustafa AtalayAgnieszka GajewskaDenisa MarginaTarja Kokkolasubject
Ultraviolet RaysClinical Chemistry TestsEnzyme-Linked Immunosorbent AssayIsoprostanesmedicine.disease_causeF2-isoprostanesSensitivity and SpecificityBiochemistryHigh-performance liquid chromatographyMass Spectrometry4-HydroxynonenalLipid peroxidationPlasmachemistry.chemical_compoundIn vivoMalondialdehydemedicineHumansChromatography High Pressure LiquidAldehydesChromatographyChemistryReproducibility of Resultsoxidative stress; F2-Isoprostanes; 4.-hydroxynonenal; malondialdehydeGeneral MedicineOxidative stress; F2-isoprostanes; 4-hydroxynonenal; malondialdehydeMalondialdehydeIsoprostanes4-hydroxynonenalF2-IsoprostanesBiochemistryOxidative stressLipid PeroxidationOxidative stressChromatography Liquiddescription
Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F2-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity. © 2010 Informa UK, Ltd.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 |