Characterization of the Breast Cancer Marker Candidate LAG3 Protein in Human Plasma by Direct SPRi-MALDI-MS Analysis from Antibody Arrays

6533b7d8fe1ef96bd126a084

RESEARCH PRODUCT

Characterization of the Breast Cancer Marker Candidate LAG3 Protein in Human Plasma by Direct SPRi-MALDI-MS Analysis from Antibody Arrays

Fabien Remy-martin Marven El Osta G. Lucchi Rabah Zeggari Therese Leblois S. Bellon E. Ly-morin C. Frydman D. Suckau Patrick Ducoroy Wilfrid Boireau

subject

[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics

description

Proteomics plays an important role in biomarker discovery as prerequisite for later utilization. In this study, we coupled Surface Plasmon Resonance imaging (SPRi) with MALDI-TOF mass spectrometry to permit the multiplexed quantification of binding by SPRi and the molecular characterization of interacting partners by subsequent MS analysis. Hyphenation of the two technologies is applied to the detection and characterization of LAG3 (potential breast cancer biomarker) in human serum1.

year	journal	country	edition	language
2013-01-01

https://hal.archives-ouvertes.fr/hal-00967060

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