0000000000315215
AUTHOR
Fabien Remy-martin
showing 2 related works from this author
Characterization of the Breast Cancer Marker Candidate LAG3 Protein in Human Plasma by Direct SPRi-MALDI-MS Analysis from Antibody Arrays
2013
Proteomics plays an important role in biomarker discovery as prerequisite for later utilization. In this study, we coupled Surface Plasmon Resonance imaging (SPRi) with MALDI-TOF mass spectrometry to permit the multiplexed quantification of binding by SPRi and the molecular characterization of interacting partners by subsequent MS analysis. Hyphenation of the two technologies is applied to the detection and characterization of LAG3 (potential breast cancer biomarker) in human serum1.
Automated Cancer Marker Characterization in Human Plasma Using SUrface PLASMON Resonance in Array combined with Mass Spectrometry (SUPRA-MS)
2012
International audience; The combination of Surface Plasmon Resonance technology with Mass Spectrometry becomes a key method for the characterization of targeted proteins in the fields of diagnosis and functional proteomics. We demonstrated in this work the ability of our SPRi-chip to capture targeted protein in biological fluids and in situ analyze by MS and MS/MS modes through automated procedure to go beyond classical immunoassays. Here, we established a proof of concept of SUPRA-MS for the detection, the identification and the characterization of a potential breast cancer marker.