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RESEARCH PRODUCT
Extraction of Enzymes from Tissues, Cells and Cell-Organelles
Gunter M. Rothesubject
biologyChemistryMicroorganismSonicationExtraction (chemistry)Golgi apparatusbiology.organism_classificationCell wallsymbols.namesakeBiochemistryOrganellesymbolsHomogenizerBacteriadescription
Microorganisms, such as bacteria, algae, moulds and others, are ruptured by sonication, by passage through a French press [1, 2] (Fig. 2.1) or a Manton-Gaulin homogenizer [3], by blending with glass beads [4], or by digesting the cell walls enzymically [5]. Extract preparation is preferably performed in the cold (+4 °C).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-01-01 |