6533b7d8fe1ef96bd126a39d
RESEARCH PRODUCT
Novel Immune TiO2 Photoluminescence Biosensors for Leucosis Detection
Arnolds UbelisA. KusevitchValentyn SmyntynaJ. LubchukSergey GevelukJ. SpigulisN. StarodubI. DoychoaJ. BukRoman ViterIgor KonupJ. DuchoslavN. SlishikAlla Tereshchenkosubject
PhotoluminescenceMaterials scienceTio2 nanoparticlesAnalytical chemistryleucosis detectionGeneral MedicinePhotochemistryLeucosisimmune biosensorsAdsorptionImmune systemExcited statephotoluminescenceBiosensorEngineering(all)TiO2 nanostructuresdescription
Abstract Novel immune photoluminescent biosensor, based on TiO2 nanoparticles, for retroviral leucosis detection has been developed. The photoluminescence spectra were excited by solid state laser with wavelength 355 nm and measured in the range of 370-800 nm. Original photoluminescence spectrum of TiO2 nanoparticles showed wide maximum at 515 nm. The biosensitive layer was formed by immobilization of retroviral leucosis antigens on the surface of TiO2 nanoparticles. Immobilization of antigens on TiO2 surface led to UV-shift of photoluminescence spectrum and increase of PL intensity. The response to different concentrations of retroviral leucosis antibodies has been measured. The decrease of spectrum intensity and IR-shift were observed after antibodies adsorption on biosensor surface. The experimental dependences of maximum shift and intensity changes versus antibodies concentration were obtained.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 | Procedia Engineering |