6533b7d8fe1ef96bd126a3aa

RESEARCH PRODUCT

An old method for good new cells

A.i. Lo MonteMaria Concetta GiovialeC. LombardoGiuseppe Damiano

subject

MalePathologymedicine.medical_specialtyAdenosineCell SurvivalAllopurinolAntigens CD19Organ Preservation Solutionsviable cellsmicro-surgical in-situ perfusionpancreasafter exsanguinationRaffinosemedicineAnimalsFicollInsulinViaspanAorta AbdominalRats WistarPancreasTransplantationbusiness.industryLeft Common Iliac ArteryGlutathionePerfusionTransplantationSettore MED/18 - Chirurgia Generalemedicine.anatomical_structureFicoll gradientFemaleSurgeryPancreasbusinessPerfusion

description

The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).

yearjournalcountryeditionlanguage
2009-05-01
10.1016/j.transproceed.2009.03.078http://hdl.handle.net/10447/35938