6533b7d8fe1ef96bd126a3c3
RESEARCH PRODUCT
Kinetics of antibodies and antigens in serum of mice experimentally infected with echinostoma caproni (trematoda: echinostomatidae)
José-guillermo EstebanCarla Muñoz-antoliAntonio MarcillaRafael ToledoBernard FriedAna Espertsubject
MaleAntibodies HelminthEnzyme-Linked Immunosorbent AssayEchinostoma caproniImmunoglobulin GIntestinal absorptionAntibodies:CIENCIAS DE LA VIDA [UNESCO]MicrobiologyMiceAntigenEchinostomaUNESCO::CIENCIAS DE LA VIDAAnimalsAntigensEcology Evolution Behavior and SystematicsEchinostomiasisMice Inbred ICRbiologyBiomphalariaAntibody titer:CIENCIAS DE LA VIDA::Biología animal (Zoología) ::Parasitología animal [UNESCO]biology.organism_classificationUNESCO::CIENCIAS DE LA VIDA::Biología animal (Zoología) ::Parasitología animalDisease Models AnimalKineticsPolyclonal antibodiesAntibodies ; Antigens ; Echinostoma caproniAntigens HelminthImmunoglobulin GImmunologybiology.proteinParasitologyTrematodaEchinostomaAntibodydescription
The present study reports on the kinetics of antibodies and antigens in serum of mice experimentally infected with 75 metacercariae of Echinostoma caproni during the first 12 wk postinfection (wpi). Antibody titers in the serum of mice were determined by an indirect enzyme-linked immunosorbent assay (ELISA) using excretory/secretory (ES) antigens of E. caproni. The early detection of antibodies against ES antigens of E. caproni is feasible using indirect ELISA. Mice developed significant antibody responses at 2 wpi, and the values progressively increased until the end of the experiment. This may be related to the intestinal absorption of adult worm antigens that induces humoral responses. The presence of E. caproni circulating antigens was determined by a capture ELISA based on polyclonal rabbit antibodies against ES antigens of E. caproni. High levels of seroantigens in mice were detected by 1–2 wpi, probably because of the local inflammatory responses in mice induced by the adult worms. A drop in circulating antigen levels was observed at 9 wpi, which could reflect changes in the intestinal tissues over the course of the infection. Toledo Navarro, Rafael, Rafael.Toledo@uv.es ; Espert Fernandez, Ana M., Ana.M.Espert@uv.es ; Marcilla Diaz, Antonio, Antonio.Marcilla@uv.es ; Esteban Sanchis, Jose Guillermo, Jguillermo.Esteban@uv.es
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-01-01 |