6533b7d8fe1ef96bd126ae8e

RESEARCH PRODUCT

Use of voltage gradient gel electrophoresis in apoptotic DNA analysis

Vincenzo IzzoMaria Rita AsaroGiusi I Forte 1Rainer Barbieri

subject

Gel electrophoresisInsectaChromatographyGel electrophoresis of nucleic acidsChemistryOrganic ChemistryApoptosisDNAGeneral MedicineFractionationBiochemistryAnalytical ChemistryElectrophoresischemistry.chemical_compoundElectricityMolecular-weight size markerPulsed-field gel electrophoresisAnimalsAgaroseElectrophoresis Polyacrylamide GelDNA

description

In this paper the use of voltage gradient gel electrophoresis (VGGE) in the electrophoretic analysis of apoptotic DNAs is described. The peculiarity of VGGE fractionation in enhancing DNA bands in the gel by reducing their thickness was used to obtain a rapid, more selective and higher-quality electrophoretic fractionation of apoptotic DNA with respect to conventional electrophoresis. The use of VGGE fractionations also allowed a reduced amount of DNA to be used to detect a characteristic apoptotic DNA ladder pattern, in a lower agarose gel concentration, with respect to conventional electrophoretic fractionation

yearjournalcountryeditionlanguage
2000-09-29Journal of Chromatography A
https://doi.org/10.1016/s0021-9673(00)00602-6