6533b7d8fe1ef96bd126b72c

RESEARCH PRODUCT

Primary ciliary dyskinesia assessment by means of optical flow analysis of phase-contrast microscopy images

David MoratalManuel De La MataJulio CortijoMiguel ArmengotEduardo ParrillaJosé Manuel Sánchez-vílchezJaime RieraJosé L. Hueso

subject

medicine.medical_specialtyChronic bronchitisPhase contrast microscopyOptical flowBeat (acoustics)Health InformaticsSensitivity and SpecificityPattern Recognition Automatedlaw.inventionTECNOLOGIA ELECTRONICAPrimary ciliary dyskinesialawOphthalmologyImage Interpretation Computer-AssistedMicroscopymedicineHumansMicroscopy Phase-ContrastRadiology Nuclear Medicine and imagingPrimary ciliary dyskinesiaMicroscopy VideoBronchiectasisRadiological and Ultrasound Technologybusiness.industryCiliumOptical flowActive contoursReproducibility of ResultsAnatomyImage Enhancementmedicine.diseaseComputer Graphics and Computer-Aided DesignCell TrackingSubtraction TechniqueFISICA APLICADABeat frequencyComputer Vision and Pattern RecognitionbusinessMATEMATICA APLICADAAlgorithmsFourier-Mellin transformCiliary Motility Disorders

description

Primary ciliary dyskinesia implies cilia with defective or total absence of motility, which may result in sinusitis, chronic bronchitis, bronchiectasis and male infertility. Diagnosis can be difficult and is based on an abnormal ciliary beat frequency (CBF) and beat pattern. In this paper, we present a method to determine CBF of isolated cells through the analysis of phase-contrast microscopy images, estimating cilia motion by means of an optical flow algorithm. After having analyzed 28 image sequences (14 with a normal beat pattern and 14 with a dyskinetic pattern), the normal group presented a CBF of 5.2 +/- 1.6 Hz, while the dyskinetic patients presented a 1.9 +/- 0.9 Hz CBF. The cutoff value to classify a dyskinetic specimen was set to 3.45 Hz (sensitivity 0.86, specificity 0.93). The presented methodology has provided excellent results to objectively diagnose PCD.

yearjournalcountryeditionlanguage
2014-04-01
10.1016/j.compmedimag.2013.12.010https://dx.doi.org/10.1016/j.compmedimag.2013.12.010