6533b7d8fe1ef96bd126b76b
RESEARCH PRODUCT
Influence of spontaneous hypertension on n-3 delta-6-desaturase activity and fatty acid composition of rat hepatocytes.
Michel NarceJean-pierre PoissonJoseph GrestiMarie-claire DelachambrePhilippe Asdrubalsubject
Fatty Acid Desaturasesmedicine.medical_specialtyClinical chemistryClinical BiochemistryLinoleoyl-CoA DesaturaseRats Inbred WKYSpontaneously hypertensive ratInternal medicineRats Inbred SHRmedicineAnimalsMolecular Biologychemistry.chemical_classificationbiologyChemistryFatty AcidsFatty acidCell BiologyGeneral MedicineDelta-6-desaturaseEnzyme assayRatsEnzyme Activationmedicine.anatomical_structureEndocrinologyEicosanoidLiverHepatocyteHypertensionbiology.proteinPolyunsaturated fatty aciddescription
The first and rate limiting step in the conversion of alpha-linolenic acid is catalyzed by the delta-6-desaturase enzyme. The activity of such an enzyme was studied in order to investigate the n-3 Polyunsaturated Fatty Acid biogenesis during hypertension. Rat isolated hepatocyte n-3 delta-6-desaturase activity was higher in 1 month old Spontaneously Hypertensive Rats — prehypertensive period- as compared to normotensive Wistar Kyoto rats, whereas there was no significant difference at 12 months — hypertensive period-. Our data indicate no correlation between the directly measured enzyme activity and the changes in hepatocyte n-3 fatty acid compositions. The loss of hepatocyte n-3 delta-6-desaturase activity in the Spontaneously Hypertensive Rat may be a key factor in the evolution of hypertension related to aging through altering the eicosanoid balance.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-11-08 | Molecular and cellular biochemistry |