6533b7d8fe1ef96bd126b7a3
RESEARCH PRODUCT
Antiasthmatic effects of onions: Inhibition of 5-lipoxygenase and cyclooxygenase in vitro by thiosulfinates and “Cepaenes”
F. WillerW. DorschTh. BayerW. BreuHans-ulrich Wagnersubject
MaleSwineClinical BiochemistryArachidonic AcidsPharmacologyAlliumStructure-Activity RelationshipLipoxygenasemedicineAnimalsCyclooxygenase InhibitorsDisulfidesLipoxygenase InhibitorsThiosulfinateArachidonic AcidSheepbiologyPlant ExtractsChemistryCell BiologySulfinic AcidsAsthmaIn vitroBiochemistryMechanism of actionEnzyme inhibitorArachidonate 5-lipoxygenasebiology.proteinMicrosomeCyclooxygenasemedicine.symptomdescription
Nine thiosulfinates (TS) and four "Cepaenes" (CS) isolated from onions and/or synthetized by us showed dose dependent (0.25 to 100 microM) marked inhibitory effects on both cyclooxygenase (CA, tested on sheep seminal vesicle microsomes) and 5-lipoxygenase activity (LO, tested on porcine leukocytes). The following rank order of activity was observed: saturated aliphatic TS less than aromatic TS approximately alpha, beta-unsaturated TS less than CS. CS inhibited both CA and LO by more than 75% at 10 and 1 microM concentrations respectively. Most likely, these in vitro effects are responsible for antiinflammatory and antiasthmatic properties of onion extracts observed in vivo, at least in part.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-01-01 | Prostaglandins, Leukotrienes and Essential Fatty Acids |