6533b7d9fe1ef96bd126c044
RESEARCH PRODUCT
(E)-2-Cyano-3-(5′-piperidin-1-yl-2,2′-bithien-5-yl)acrylic Acid: A Fluorescent Probe for Detecting Prefibrillar Oligomers
Bruno PignataroSebastiano CataldoCosimo G. FortunaCarmela BonaccorsoFrancesco BelliaFrancesco AttanasioEnrico RizzarelliGiuseppe Musumarrasubject
AmyloidAtomic force microscopyOrganic ChemistryNative Polyacrylamide Gel ElectrophoresisFluorescencechemistry.chemical_compoundElectrophoresischemistryDynamic light scatteringBiophysicsOrganic chemistryThioflavinPhysical and Theoretical ChemistryAcrylic aciddescription
The synthesis of (E)-2-cyano-3-(5′-piperidin-1-yl-2,2′-bithien-5-yl)acrylic acid, a novel amyloid aggregation fluorescent probe, is reported. This new probe is able to monitor soluble oligomeric aggregates after 24 h, at which time Thioflavin T emission, commonly used to monitor amyloid fibril formation, remains unchanged. Atomic force microscopy, native polyacrylamide gel electrophoresis, and dynamic light scattering confirm that the earlier stages of aggregation are prefibrillar oligomeric species not possessing the amyloid structure. This new molecular scaffold expands the toolbox of fluorescent probes for the identification of prefibrillar oligomers, which is needed in studies aimed at the early detection of the soluble toxic aggregates that characterize the so-called protein misfolding diseases.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-04-29 | European Journal of Organic Chemistry |