6533b7d9fe1ef96bd126c1ab

RESEARCH PRODUCT

Seasonal variations of antimicrobial activity and chemical composition of essential oils extracted from three Citrus limon L. Burm. cultivars

Walter RandazzoP.l. San BiagioCaterina MamminaMarta MoschettiValeria GuarrasiFrancesco MarraAurora AleoLuca SettanniEristanna PalazzoloMaria GermanaGiancarlo Moschetti

subject

CitrusStaphylococcus aureusfoodborne pathogenSettore AGR/13 - Chimica AgrariaEnterobacterMicrobial Sensitivity TestsPlant ScienceSettore MED/42 - Igiene Generale E Applicatamedicine.disease_causeBiochemistryessential oilGas Chromatography-Mass SpectrometryAnalytical Chemistryantibacterial activityAnti-Infective AgentsGram-Negative BacteriaBotanyOils Volatilemedicinechemical compositionCultivarChemical compositionbiologyseasonal variationsOrganic ChemistrySalmonella entericaPathogenic bacteriaEnterobacterAntimicrobialbiology.organism_classificationListeria monocytogenesSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeHorticulturelemon fruitItalyFruitSeasonsGas chromatographyGas chromatography–mass spectrometryAntibacterial activitySettore AGR/16 - Microbiologia Agraria

description

In order to investigate the seasonal variations of antimicrobial properties and chemical composition of essential oils (EOs), three different cultivars of Citrus limon L. Burm. spp. (Femminello Santa Teresa, Monachello and Femminello Continella) were collected at 6-week intervals, from December 2012 to April 2013, for a total of four harvests. The EOs were extracted from lemon peel by hydro-distillation. The antimicrobial activity, tested by paper disc diffusion method, was evaluated against common food-related pathogenic bacteria (Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Enterobacter spp.). EOs were more effective against Gram-positive than Gram-negative bacteria at each collection time, but a strong strain dependence was evidenced. Monachello EOs showed the highest inhibition power. The chemical characterisation of the EOs performed by gas chromatography/mass spectrometry identified from 36 to 42 molecules. The chemical difference registered among samples and seasons may explain the different antimicrobial efficacies recorded.

https://dx.doi.org/10.6084/m9.figshare.960061.v2