6533b7d9fe1ef96bd126c1c4

RESEARCH PRODUCT

Plant regeneration from juvenile and adult Anthyllis cytisoides, a multipurpose leguminous shrub

Juan SeguraCarlota ZaragozaIsabel GavidiaPedro Pérez-bermúdez

subject

biologyPhysiologyved/biologyfungived/biology.organism_classification_rank.speciesfood and beveragesAnthyllisPlant ScienceAnthyllis cytisoidesbiology.organism_classificationShrubMicropropagationAxillary budShootBotanyJuvenileMultipurpose treeAgronomy and Crop Science

description

Summary Anthyllis cytisoides , a legume shrub used for afforestation and reclamation of degraded Mediterranean areas, was successfully micropropagated from expiants of juvenile (cotyledonary nodes and apical buds) and adult origin (axillary buds). Multiple shoot formation was dependent on the presence of benzyladenine in the induction medium. Of the salt formulation and expiants examined, the higher proliferation rates were obtained when axillary buds from adult plants were cultured on a modified Schenk and Hildebrandt medium. Following the preferred protocol, shoot yield reached values greater than 100 shoots per expiant. Plants were easily rooted and transplanted into greenhouse.

https://doi.org/10.1016/s0176-1617(97)80288-x