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RESEARCH PRODUCT
IL-21 Regulates the Differentiation of a Human γδ T Cell Subset Equipped with B Cell Helper Activity
Guido SireciNadia CaccamoFrancesco DieliMarco Pio La MannaMatilde TodaroGiorgio Stassisubject
Anatomy and PhysiologyImmunoglobulin delta-ChainsB CellsCellular differentiationAntibody Affinitylcsh:MedicineAdaptive Immunitychemistry.chemical_compoundWhite Blood CellsSpectrum Analysis TechniquesCell MovementAnimal CellsImmune PhysiologyMedicine and Health SciencesCytotoxic T celllcsh:ScienceImage CytometryB-LymphocytesMultidisciplinarybiologyT CellsCell DifferentiationT-Lymphocytes Helper-InducerFlow CytometryInnate ImmunityDNA-Binding Proteinsmedicine.anatomical_structureIL-21 differentiation Vγ9Vδ2 T lymphocytesSpectrophotometryCD4 AntigensProto-Oncogene Proteins c-bcl-6CytokinesMedicineCytophotometryChemokinesCellular TypesResearch ArticleCD4 antigenImmunoglobulin gamma-ChainsImmune CellsImmunologyMajor histocompatibility complexResearch and Analysis MethodsAntigenmedicineHumansCXCL13Antibody-Producing CellsBiologyB cellCell ProliferationSettore MED/04 - Patologia GeneraleCD40Blood CellsInterleukinsFluorimetrylcsh:RImmunityBiology and Life SciencesCell BiologyMolecular biologyRetractionchemistryGene Expression RegulationHumoral Immunitybiology.proteinClinical Immunologylcsh:QDevelopmental Biologydescription
Vγ9Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and display pleiotropic features. Here we report that coculture of Vγ9Vδ2 cells with phosphoantigen and IL-21 leads to selective expression of the transcription repressor Bcl-6 and polarization toward a lymphocyte subset displaying features of follicular B-helper T (T(FH)) cells. T(FH) like Vγ9Vδ2 cells have a predominant central memory (CD27(+)CD45RA(-)) phenotype and express ICOS, CD40L and CXCR5. Upon antigen activation, they secrete IL-4, IL-10 and CXCL13, and provide B-cell help for antibody production in vitro. Our findings delineate a subset of human Vγ9Vδ2 lymphocytes, which, upon interaction with IL-21-producing CD4 T(FH) cells and B cells in secondary lymphoid organs, is implicated in the production of high affinity antibodies against microbial pathogens.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 | PloS one |