Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

6533b7d9fe1ef96bd126c37d

RESEARCH PRODUCT

Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis, immune and hypoxia related genes

Susanne Jong-raadsen Jerko Hrabar Ariadna Sitjà-bobadilla Ron P. Dirks M. Carla Piazzon Jaume Pérez-sánchez Anamarija Vrbatović Ivona Mladineo Fernando Naya-català Fernando Naya-català

subject

0106 biological sciences Gill Gills Apoptosis 01 natural sciences Transcriptome Sparus aurata Gene expression 0303 health sciences High-Throughput Nucleotide Sequencing medicine.anatomical_structure Liver Helminthiasis Animal Monogenea Biotechnology Research Article Fish Proteins animal structures lcsh:QH426-470 lcsh:Biotechnology Fisheries Spleen Biology Microbiology Host-Parasite Interactions 03 medical and health sciences Immune system Illumina RNA-seq Immunity lcsh:TP248.13-248.65 Genetics medicine Autophagy Animals 14. Life underwater Platelet activation Immune response Transcriptomics 030304 developmental biology Cell Proliferation Sequence Analysis RNA Sparus aurata Sparicotyle chrysophrii Gills Monogenea Ectoparasites Illumina RNA-seq Transcriptomics Apoptosis Immune response Gene Expression Profiling Aquatic animal Sea Bream lcsh:Genetics Gene Expression Regulation Platyhelminths Sparicotyle chrysophrii Ectoparasites Spleen 010606 plant biology & botany

description

[Background] Monogenean flatworms are the main fish ectoparasites inflicting serious economic losses in aquaculture. The polyopisthocotylean Sparicotyle chrysophrii parasitizes the gills of gilthead sea bream (GSB, Sparus aurata) causing anaemia, lamellae fusion and sloughing of epithelial cells, with the consequent hypoxia, emaciation, lethargy and mortality. Currently no preventive or curative measures against this disease exist and therefore information on the host-parasite interaction is crucial to find mitigation solutions for sparicotylosis. The knowledge about gene regulation in monogenean-host models mostly comes from freshwater monopysthocotyleans and almost nothing is known about polyopisthocotyleans. The current study aims to decipher the host response at local (gills) and systemic (spleen, liver) levels in farmed GSB with a mild natural S. chrysophrii infection by transcriptomic analysis.

year	journal	country	edition	language
2019-03-11

10.1186/s12864-019-5581-9 http://hdl.handle.net/10261/178043