An asymmetric electrospun membrane for the controlled release of ciprofloxacin and FGF-2: Evaluation of antimicrobial and chemoattractant properties.

6533b7d9fe1ef96bd126c4a6

RESEARCH PRODUCT

An asymmetric electrospun membrane for the controlled release of ciprofloxacin and FGF-2: Evaluation of antimicrobial and chemoattractant properties.

Giovanna Pitarresi Fabio Salvatore Palumbo Domenico Schillaci Calogero Fiorica Gaetano Giammona Salvatore Federico Valentina Catania

subject

Materials science Polyurethanes Nanofibers Bioengineering macromolecular substances 02 engineering and technology Chemotaxis (FGF-2)Antimicrobial activity (CPX); Chemotaxis (FGF-2); Double layer electrospun membrane; Gellan gum alkyl-derivative; Polyurethanes 010402 general chemistry 01 natural sciences Polyvinyl alcohol Gellan gum alkyl-derivative Biomaterials chemistry.chemical_compound Anti-Infective Agents Ciprofloxacin Copolymer Double layer electrospun membrane Chemotactic Factors technology industry and agriculture 021001 nanoscience & nanotechnology Antimicrobial Controlled release Bandages Gellan gum Electrospinning 0104 chemical sciences Anti-Bacterial Agents Antimicrobial activity (CPX)Membrane chemistry Mechanics of Materials Settore CHIM/09 - Farmaceutico Tecnologico Applicativo Delayed-Action Preparations Biophysics Fibroblast Growth Factor 2 0210 nano-technology Layer (electronics)

description

Here, an asymmetric double-layer membrane has been designed and fabricated by electrospinning as a tool for a potential wound healing application. A hydrophobic layer has been produced by using a polyurethane-polycaprolactone (PU-PCL) copolymer and loaded with the antibacterial ciprofloxacin whereas an ion responsive hydrophilic layer has been produced by using an octyl derivative of gellan gum (GG-C8) and polyvinyl alcohol (PVA) and loaded with the growth factor FGF-2. This study investigated how the properties of this asymmetric membrane loaded with actives, were influenced by the ionotropic crosslinking of the hydrophilic layer. In particular, the treatment in DPBS and the crosslinking in CaCl2 0.1 or 1 M of the hydrophilic layer affected the release profile of the bioactive molecules allowing to modulate both the antimicrobial effect, as assayed by logarithmic reduction of the Staphylococcus aureus viable count, and the chemoattractant properties on NIH 3 T3 cell line, as assayed by scratch test and cell chemoattraction assay. © 2021 Elsevier B.V.

year	journal	country	edition	language
2021-01-01	Materials scienceengineering. C, Materials for biological applications

10.1016/j.msec.2021.112001 https://pubmed.ncbi.nlm.nih.gov/33812621