Šūnu - selex atlasīto aptamēra specifiskums in vitro

6533b7d9fe1ef96bd126c666

RESEARCH PRODUCT

Šūnu - selex atlasīto aptamēra specifiskums in vitro

subject

Aptamēri SELEX Farmācija Plūsmas citometrija Nieru karcinoma

description

Aptamēri aizvien vairāk pierāda savas spējas diagnostikā kā arī terapijā. Vēl aizvien daudzām sarežģītām slimībām trūkst terapijas vai diagnostikas līdzekļi, tādēļ aptamēri varētu būt potenciāli terapijas vai diagnostikas līdzekļi. Šajā pētījumā tika pētīti iepriekš atlasīti nieru kanāliņu epitēlija un nieru karcinomas šūnu līniju RC-124 un RCC-MF specifiski aptamēri (seq36), izmantojot attēlveidojošo plūsmas citometrijas metodi. Tika veikts aptamēru selektivitātes skrīnings pret RC-124, RCC-MF, 769-P, MCF-7, MDA-MB-231, THP-1 un HeLa šūnu līnijām. Novērojām visspēcīgāko aptamēra 36seq saistīšanos ar MDA-MB-231 šūnām, tomēr ar MCF-7 saistīšanās netika novērota, tā pat kā ar THP-1 un HeLa šūnām. Afinitātes tests tika veikts MDA-MB-231 un MCF-7 šūnām.

year	journal	country	edition	language
2020-01-01

https://dspace.lu.lv/dspace/handle/7/52067

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