6533b7d9fe1ef96bd126ca9d

RESEARCH PRODUCT

Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections

Manish Mani SubramaniamNina GaleRosa NogueraSamuel NavarroMarta Piqueras

subject

Bacterial artificial chromosomechemistry.chemical_compoundFormalin fixed paraffin embeddedmedicine.diagnostic_testProtein digestionChemistryHybridization probemedicineFish <Actinopterygii>Gene rearrangementMolecular biologyDNAFluorescence in situ hybridization

description

Fluorescence In Situ Hybridization (FISH) is a powerful technique for localizing specific DNA targets directly in the fixed tissue or cells. Bacterial artificial chromosome (BAC) as well as commercial probes, which could be supplied ready for use or concentrated and must be diluted following the manufacturers instructions, can be used. The technique requires 2 days, as an overnight incubation of the FISH probes is needed for optimal hybridization. The critical steps include deparaffinization of tissue sections, optimal pretreatment (target retrieval and protein digestion), and probe hybridization. In this chapter, the described FISH protocol provides a methodology for analyzing the cytogenetic alterations in formalin-fixed-paraffin-embedded (FFPE) tissue sections.

yearjournalcountryeditionlanguage
2011-01-01
https://doi.org/10.1007/978-3-642-17890-0_34