6533b7d9fe1ef96bd126cace
RESEARCH PRODUCT
Flow-injection spectrophotometric determination of amino acids based on an immobilised copper(II) -zincon system
J. Martínez CalatayudL. Lahuerta Zamorasubject
chemistry.chemical_classificationChromatographychemistryCalibration curveGlycineRelative standard deviationEnvironmental Chemistrychemistry.chemical_elementBiochemistryCopperSpectroscopyAnalytical ChemistryAmino aciddescription
Abstract The flow-injection spectrophotometric determination of different amino acids was carried out by reaction with copper(II) ions entrapped in a polymeric material and filling a packed-bed reactor; the released copper(II), complexed with the amino acid, reacted with zincon in a basic medium producing a blue colour that was monitored at 600 nm. The method was applied to determine the contents of different amino acids in pharmaceutical formulations. The calibration graph for glycine was linear over the range 0.5–20 μg ml −1 with a relative standard deviation of 0.8% ( n = 6) at 10 μg ml −1 and a sample throughput of 108 h −1 .
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-09-01 | Analytica Chimica Acta |