6533b7d9fe1ef96bd126cc68

RESEARCH PRODUCT

Assessing Chronological Aging in Saccharomyces cerevisiae

Mario G. MirisolaMin WeiJia HuValter D. Longo

subject

Cell NucleusGenome instabilityGeneticsMutation rateeducation.field_of_studyTime Factorsbiologyved/biologySaccharomyces cerevisiaeved/biology.organism_classification_rank.speciesPopulationFungal geneticsWaterSaccharomyces cerevisiaebiology.organism_classificationArticleGenomic InstabilityYeastCulture Mediayeast genetics aging chronological agingMutation RateDNA FungalModel organismeducationGene

description

Saccharomyces cerevisiae is one of the most studied model organisms for the identification of genes and mechanisms that affect aging. The chronological lifespan (CLS) assay, which monitors the survival of a non-dividing population, is one of the two methods to study aging in yeast. To eliminate potential artifacts and identify genes and signaling pathways that may also affect aging in higher eukaryotes, it is important to determine CLS by multiple methods. Here, we describe these methods as well as the assays to study macromolecular damage during aging in yeast, with a focus on genomic instability.

yearjournalcountryeditionlanguage
2012-11-01
https://doi.org/10.1007/978-1-62703-239-1_30