6533b7d9fe1ef96bd126cdf0
RESEARCH PRODUCT
Bifunctional viscous nanovesicles co-loaded with resveratrol and gallic acid for skin protection against microbial and oxidative injuries.
Justina VitonyteJosé Esteban PerisGermano OrrùIris UsachMaria Letizia MancaCarla CaddeoDonatella ValentiAnna Maria FaddaMaria ManconiXavier Fernàndez-busquetsMaría MatosAmparo NácherGemma Gutiérrezsubject
0301 basic medicineKeratinocytesCell SurvivalSwinePharmaceutical Science02 engineering and technologyResveratrolIn Vitro Techniquesmedicine.disease_causeSkin DiseasesAntioxidants03 medical and health scienceschemistry.chemical_compoundDrug StabilityGallic AcidStilbenesGlycerolmedicineAnimalsHumansGallic acidPhenolsParticle SizeBifunctionalPhospholipidsLiposomeChromatographyViscosityVesicleGeneral MedicineSkin Diseases BacterialFibroblasts021001 nanoscience & nanotechnology030104 developmental biologychemistryAnimals NewbornResveratrolLiposomesAnti-Infective Agents Local0210 nano-technologyOxidative stressBiotechnologydescription
Resveratrol and gallic acid were co-loaded in phospholipid vesicles aiming at protecting the skin from external injuries, such as oxidative stress and microbial infections. Liposomes were prepared using biocompatible phospholipids dispersed in water. To improve vesicle stability and applicability, the phospholipids and the phenols were dispersed in water/propylene glycol or water/glycerol, thus obtaining PEVs and glycerosomes, respectively. The vesicles were characterized by size, morphology, physical stability, and their therapeutic efficacy was investigated in vitro. The vesicles were spherical, unilamellar and small in size: liposomes and glycerosomes were around 70nm in diameter, while PEVs were larger (∼170nm). The presence of propylene glycol or glycerol increased the viscosity of the vesicle systems, positively affecting their stability. The ability of the vesicles to promote the accumulation of the phenols (especially gallic acid) in the skin was demonstrated, as well as their low toxicity and great ability to protect keratinocytes and fibroblasts from oxidative damage. Additionally, an improvement of the antimicrobial activity of the phenols was shown against different skin pathogens. The co-loading of resveratrol and gallic acid in modified phospholipid vesicles represents an innovative, bifunctional tool for preventing and treating skin affections.
year | journal | country | edition | language |
---|---|---|---|---|
2017-05-01 | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V |