6533b7d9fe1ef96bd126ce53
RESEARCH PRODUCT
Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil-miscible KOH/propanol/methanol medium.
Carla Rosane Barboza MendonçaCarla Rosane Barboza MendonçaClarisse Maria Sartori PiatnickiClara Ismeria Damiani BicaGuillermo Ramis-ramosErnesto F. Simó-alfonsosubject
food.ingredientChromatographyCapillary actionPotassium CompoundsMethanolClinical Biochemistryfood and beveragesElectrophoresis CapillaryWheat germElectrolyte1-PropanolBiochemistrySoybean oilAnalytical ChemistryPropanolchemistry.chemical_compoundCapillary electrophoresisfoodchemistryPhenolsSolubilityYield (chemistry)HydroxidesPlant OilsMethanoldescription
The separation of hydroxyaromatic compounds in vegetable oils, including synthetic antioxidants (3-tert-butyl-4-hydroxyanisol and 2,6-di-tert-butyl-4-hydroxytoluene), E-vitamers and other natural oil components, by nonaqueous capillary electrophoresis in an oil-miscible background electrolyte (BGE) was investigated. The BGE contained 40 mM KOH in a methanol/1-propanol (PrOH) mixture (15:85 v/v). The oil samples were 1:1 diluted with PrOH and directly injected in the capillary. Under negative polarity (cathode at the injection end), the anionic solutes moved faster than the electroosmotic flow, being well-resolved among them and from the triacylglycerols. Using virgin palm, extra virgin olive, wheat germ, virgin soybean and other oils, the capability of the procedure to quickly yield a characteristic profile of the biophenols present in the sample was demonstrated. The alpha-, (beta + gamma)- (as unresolved pair) and delta-tocopherols of a soybean oil sample were quantified.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-09-01 | Electrophoresis |