6533b7d9fe1ef96bd126cea1
RESEARCH PRODUCT
Reply to "Heparan Sulfate in Baculovirus Binding and Entry of Mammalian Cells"
Varpu MarjomäkiJohanna P. LaakkonenPaula TurkkiKaisa-emilia MakkonenSeppo Ylä-herttualaKari J. Airennesubject
BaculoviridaevirusesImmunologyVirus AttachmentGene deliveryMicrobiologySyndecan 1chemistry.chemical_compoundSulfationVirologymedicineHumansReceptorLetters to the Editorchemistry.chemical_classificationproteiinibiologyHeparan sulfateHeparinVirus Internalizationbiology.organism_classificationMolecular biologychemistryInsect ScienceReceptors VirusSyndecan-1GlycoproteinproteinBaculoviridaemedicine.drugdescription
(1), we investigated the interaction ofbaculovirus and mammalian cell surface heparan sulfate pro-teoglycans (HSPG). The data show that baculovirus requiresHSPG sulfation, particularly N- and 6-O-sulfation, to bind andtransduce mammalian cells. We also show that baculovirus asso-ciates specifically with syndecan-1 (SDC-1) but not with othersyndecans or glypicans.As discussed in the article, HS has previously been shown to beinvolved in glycoprotein 64 (gp64)-mediated baculovirus bindingonto mammalian cells. Heparin and heparinase I and II treatmentof cells have also been shown to prevent the virus binding (2, 3).The role of HS in baculovirus entry was further studied in ourarticle (1). Binding and baculovirus-mediated gene delivery wereseen to decrease after NaClO
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-04-15 |