6533b7d9fe1ef96bd126ceef
RESEARCH PRODUCT
Determination of α-Tocopherol in Plasma by High Performance Liquid Chromatography with Fluorescence Detection and Stability of α-Tocopherol under Different Conditions
Till HumbertUlrich FauthAndreas Rümelinsubject
ChromatographyChemistryalpha-TocopherolAnalytical chemistryReproducibility of Resultsfood and beveragesLiquid nitrogenHigh-performance liquid chromatographyFluorescenceFluorescence spectroscopyTocofersolanSpecimen Handlingchemistry.chemical_compoundSpectrometry FluorescenceDrug DiscoveryHumansSample preparationTocopherolQuantitative analysis (chemistry)Chromatography High Pressure Liquiddescription
In order to study the clinical effects of the antioxidant alpha-tocopherol (tocofersolan, CAS 30999-06-5) a simple, robust, sensitive and reliable high performance liquid chromatography (HPLC) method using fluorescence detection for the daily measurement of alpha-tocopherol concentration in plasma is described. Several methods of HPLC analysis using fluorescence techniques are published, however, investigations of the stability of alpha-tocopherol during sample preparation under different conditions are relevant in the routine measurements and have to date not been reported. Therefore a modified method, both sensitive and robust, qualified for the day-by-day measurement of alpha-tocopherol in plasma was established and the stability of alpha-tocopherol during sample preparation was analysed under different conditions. This method enables the complete separation of alpha-tocopherol from other compounds within 14 min. In addition, investigations on the stability of alpha-tocopherol during sample preparation showed the necessity of rapid sample preparation without delay. Prepared samples can be stored in liquid nitrogen for at least 56 days.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-12-25 | Arzneimittelforschung |