6533b7d9fe1ef96bd126cef3
RESEARCH PRODUCT
Electrical Pumping of Potassium Ions Against an External Concentration Gradient in a Biological Ion Channel
Salvador MafeMaría Queralt-martínElena García-giménezAntonio AlcarazVicente M. AguilellaPatricio Ramirezsubject
MicroorganismosPhysics and Astronomy (miscellaneous)PotassiumSeparaciónRatchetAnalytical chemistryBiophysicschemistry.chemical_elementRATCHETPotassium ionsFenómenos bioeléctricosIonSeparationBioelelectric phenomenaRectificationPORINVOLTAGEPotasiomicroorganismsIon channelChemistrypHNANOFLUIDIC DIODEBiomembrane transportIones positivosRECTIFICATIONOMPFTransporte de biomembranasCanales iónicosNanoporeSELECTIVITYPositive ionsFISICA APLICADAIon channelsPotassiumSelectivityConcentration gradientCommunication channelNANOPORESdescription
We show experimentally and theoretically that significant currents can be obtained with a biological ion channel, the OmpF porin of Escherichia coli, using zero-average potentials as driving forces. The channel rectifying properties can be used to pump potassium ions against an external concentration gradient under asymmetric pH conditions. The results are discussed in terms of the ionic selectivity and rectification ratio of the channel. The physical concepts involved may be applied to separation processes with synthetic nanopores and to bioelectrical phenomena. (C) 2013 AIP Publishing LLC.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-07-22 |