6533b7d9fe1ef96bd126cf1f
RESEARCH PRODUCT
Differential Effects of Nonhydroxylated Flavonoids as Inducers of Cytochrome P450 1A and 2B Isozymes in Rat Liver
Norbert LatruffeMarie-louise MillerMarie-chantal Canivenc-lavierJ. LeclercMarie-hélène SiessM. SuschetetMarc Bentejacsubject
MaleCytochromeBlotting WesternMolecular Sequence Data[SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chainToxicologyFlavonesIsozymeTangeretinchemistry.chemical_compoundCytochrome P-450 Enzyme SystemAnimalsRNA MessengerRats WistarEnzyme inducerFlavonoidsPharmacologychemistry.chemical_classificationBase SequencebiologyCYP1A2Cytochrome P450Blotting NorthernFlavonesRatsIsoenzymesLiverchemistryBiochemistryEnzyme InductionFlavanonesMicrosomes Liverbiology.proteinFlavanonedescription
Flavanone, flavone, and tangeretin differentially affected the activities of cytochrome P540 1A and 2B isozymes in rat liver. Flavone and, to a lesser extent, tangeretin, increased activities of ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, and pentoxyresorufin O-dealkylase (PROD), whereas flavanone mainly enhanced PROD activity. Immunoblot analysis indicated that flavone and tangeretin increased cytochrome P450 1A1, 1A2, and 2B1,2 forms, whereas flavanone only enhanced the cytochrome P450 2B isozymes. Northern blot study showed that flavone and tangeretin increased the level of the cytochrome P450 1A2 mRNAs. The concentration of the other mRNAs were slightly or not affected by flavonoids. These results suggest that the induction of P450 1A2 by flavone and tangeretin might involve a transcriptional and/or post-transcriptional mechanism.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-02-01 | Toxicology and Applied Pharmacology |