6533b7d9fe1ef96bd126d57f

RESEARCH PRODUCT

Axial resolution in two-color excitation fluorescence microscopy by phase-only binary apodization

Jesús LancisA. PonsMaría Teresa Caballero CaballeroManuel Martínez-corralPedro Andrés

subject

Point spread functionMaterials sciencebusiness.industryExcitation filterPhase (waves)Filter (signal processing)Atomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsOpticsApodizationFluorescence microscopeElectrical and Electronic EngineeringPhysical and Theoretical ChemistrybusinessOptical filterExcitation

description

We study the effect of a kind of binary phase-only filters, the Toraldo filters, in two-color excitation fluorescence microscopy. We show that by simple insertion of a properly designed Toraldo filter in one of the illumination arms the axial resolution of the system is significantly improved. Specifically, the main peak of the point spread function is narrowed by 22% along the axial direction.

yearjournalcountryeditionlanguage
2005-02-01Optics Communications
https://doi.org/10.1016/j.optcom.2004.11.035