6533b7d9fe1ef96bd126d5d9

RESEARCH PRODUCT

Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor

Ari WaismanJulia BruttgerFlorent GinhouxFlorent GinhouxMaja KiticPeter See

subject

0301 basic medicineDiphtheria toxinMacrophage Colony-Stimulating Factor 1 Receptoreducation.field_of_studyMicrogliaPopulationBiologyCell biology03 medical and health sciences030104 developmental biology0302 clinical medicinemedicine.anatomical_structureGenetic modelBlocking antibodyCX3CR1medicineeducationReceptor030217 neurology & neurosurgery

description

Microglia are the main population of macrophage residing in the central nervous system (CNS). Depletion experiments gave important insights into the physiology and function of microglia in healthy and diseased CNS. Ablation of microglia can be achieved by application of pharmacological or genetic tools. Here, we describe two approaches to ablate microglia: an efficient genetic model that utilizes DTRMG mouse line that has diphtheria toxin receptor (DTR) expression regulated by the promoter activity of the fractalkine receptor (CX3CR1) gene, and a pharmacological model that utilizes the blocking of macrophage colony-stimulating factor 1 receptor (CSF-1R) with a blocking antibody. Both the administration of systemic diphtheria toxin or anti-CSF-1R blocking antibody result in highly efficient and reversible depletion of microglia population in the CNS, which can be easily assessed by flow cytometry.

yearjournalcountryeditionlanguage
2019-01-01
https://doi.org/10.1007/978-1-4939-9658-2_16