6533b7d9fe1ef96bd126d62d
RESEARCH PRODUCT
Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria
Andreas DiefenbachAndreas DiefenbachKonrad GronkeKonrad GronkeMichael Kofoed-nielsenMichael Kofoed-nielsensubject
0301 basic medicineLamina propriaInnate immune systemmedicine.diagnostic_testInnate lymphoid cellBiologyFlow cytometry03 medical and health sciences030104 developmental biology0302 clinical medicinemedicine.anatomical_structureImmune systemIntestinal mucosaImmunityImmunologymedicineHomeostasis030215 immunologydescription
The intestinal mucosa constitutes the biggest surface area of the body. It is constantly challenged by bacteria, commensal and pathogenic, protozoa, and food-derived irritants. In order to maintain homeostasis, a complex network of signaling circuits has evolved that includes contributions of immune cells. In recent years a subset of lymphocytes, which belong to the innate immune system, has caught particular attention. These so-called innate lymphoid cells (ILC) reside within the lamina propria of the small and large intestines and rapidly respond to environmental challenges. They provide immunity to various types of infections but may also contribute to organ homeostasis as they produce factors acting on epithelial cells thereby enhancing barrier integrity. Here, we describe how these cells can be isolated from their environment and provide an in-depth protocol how to visualize the various ILC subsets by flow cytometry.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-01 |