6533b7dafe1ef96bd126d86e
RESEARCH PRODUCT
Cloning and sequencing of the gene encoding α-acetolactate decarboxylase fromLeuconostoc oenos
Christophe MonnetCharles DivièsJean-françois CavinDominique GarmynJean GuzzoBrigitte Martineausubject
DNA BacterialCarboxy-LyasesOperonMolecular Sequence DataRestriction MappingBiologyMicrobiologyGene Expression Regulation EnzymologicGeneticsLeuconostocGenomic libraryCloning MolecularMolecular BiologyGeneGeneticsCloningSequence Homology Amino AcidNucleic acid sequenceGene Expression Regulation BacterialSequence Analysis DNABlotting Northernbiology.organism_classificationAcetolactate decarboxylaseAcetolactate SynthaseRNA BacterialOpen reading framePhenotypeBiochemistryGenes BacterialLactatesLeuconostocdescription
The alsD gene encoding alpha-acetolactate decarboxylase was isolated from a genomic library of Leuconostoc oenos, using a screening procedure developed on microtiter plates. The nucleotide sequence of alsD encodes a putative protein of 239 amino acids showing significant similarity with other bacterial alpha-acetolactate decarboxylases. Upstream from alsD lies an open reading frame (alsS) which is highly similar to bacterial genes coding for catabolic alpha-acetolactate synthases. Northern (RNA) blotting analyses indicated the presence of a 2.4-kb dicistronic transcript of alsS and alsD. This suggests that the alsS and alsD genes are organized in a single operon.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-12-01 | FEMS Microbiology Letters |