6533b7dafe1ef96bd126e178

RESEARCH PRODUCT

Electrochemical detection of nitric oxide in plant cell suspensions

David WendehenneDavid WendehenneAngélique Besson-bardAngélique Besson-bardFethi BediouiSophie Griveau

subject

0106 biological sciences0301 basic medicinehome-made electrodeChemistry[SDV]Life Sciences [q-bio]fungichemistry.chemical_elementElectrochemical detectionPlant cellElectrochemistry01 natural sciencesNitric oxide03 medical and health scienceschemistry.chemical_compound030104 developmental biologyChemical engineeringnitric oxideplant cell suspensionselectrochemical detection[SDE]Environmental Sciences[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyIridiumPlatinumSelectivity010606 plant biology & botany

description

SPE IPM UB; Nitric oxide is a hydrophobic radical acting as a physiological mediator in plants. Because of its unique properties, the detection of NO in plant tissues and cell suspensions remains a challenge. For this purpose, several techniques are used, each having certain advantages and limitations such as interferences with other species, questionable sensitivity, and/or selectivity or ex situ measurement. Here we describe a very attractive approach for tracking NO in plant cell suspensions using a NO-sensitive homemade platinum/iridium-based electrochemical microsensor. This method constitutes the absolute real-time proof of the production of free NO in physiological conditions.

yearjournalcountryeditionlanguage
2016-01-01
10.1007/978-1-4939-3600-7_11https://hal.inrae.fr/hal-02800189