6533b7dafe1ef96bd126e371

RESEARCH PRODUCT

Label-free wavelength and phase detection based SMS fiber immunosensors optimized with cladding etching

subject

description

The performance of E-SMS (Etched Singlemode-Multimode-Singlemode) optical fiber structures as immunosensors has been assessed by the implementation of antibody/antigen immunoassays. Through this procedure it has been proven that E-SMS structures are effective and suitable optical platforms for label-free biosensing. Using the phase shift and tracking the wavelength response it was found that the fabricated E-SMS devices exhibited limits of detection (LOD) down up to concentrations of 0.2mg/L of antigens in solution. This was achieved by coating the E-SMS with an antibody-based biolayer (goat IgG) that is able to determine the presence of anti-goat IgG antigen. Both a wavelength detection and a fast Fourier transform (FFT) analysis technique were used to perform this analysis. The FFT method showed similar results to those observed with the most traditional wavelength analysis, but with the advantage of a simpler detection system that makes unnecessary the use of sophisticated optical interrogators. This work was supported in part by the Spanish Ministry of Education and Science - FEDER TEC 2016- 78047 and by the Government of Navarre through its projects with references: 2016/PI044, 2016/PC025 and 2016/PC026. This work was supported in part by the Colombian Administrative Department of Science, Technology and Innovation - Colciencias, through the Program for national doctorates, calling 617 of 2013, and by the project “BIOSENSORES A FIBRA ÓPTICA PARA LA DETECCIÓN RÁPIDA DE ENFERMEDADES” supported by the Universidad Nacional de Colombia (Hermes code 40834).