6533b7dafe1ef96bd126e382
RESEARCH PRODUCT
Staining mitochondria in Saccharomyces cerevisiae.
Jürgen Reisssubject
ChromatographyTime FactorsStaining and LabelingJanus Green BSaccharomyces cerevisiaeBiologyBuffersHydrogen-Ion Concentrationbiology.organism_classificationStainLuxol fast blue stainStainingMitochondriaElectron Transport Complex IVchemistry.chemical_compoundSaccharomyceschemistryBiochemistryDistilled waterbiology.proteinMethodsCytochrome c oxidaseAnatomyColoring AgentsIncubationdescription
After testing various procedures (amidoblack 10B, acid fuchsin-methyl blue, Luxol fast blue MBS-phloxine, toluidine blue O, Jams green B and pinacyanol), three stains can be recommended for staining both types of mitochondria (globose and threadlike) in the cells of Saccharomyces cerevisiae: (1) 0.1% solution of amidoblack 10B in citrate buffer (pH 3.0) for 10 min; (2) 0.01% solution of toluidine blue O in phosphate buffer (pH 6.0) for 30 min; (3) 0.01% solution of Janus green B in distilled water (pH 5.6) for 30 min. The latter stain is most specific because its staining reaction depends upon the action of the mitochondrial enzyme cytochrome c oxidase. Yet, low concentrations and short incubation periods must be applied to avoid poisoning of the cell metabolism.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1969-01-01 | Stain technology |