6533b7dafe1ef96bd126eb33
RESEARCH PRODUCT
Modulation of mitochondriotropic properties of cyanine dyes by in organello copper-free click reaction
Hans-jürgen ButtKaloian KoynovMarkus HirschTom BrownInka NegwerInka NegwerKalina PenevaStefka KaloyanovaMark Helmsubject
0301 basic medicinechemistry.chemical_elementBiochemistryCell Line03 medical and health scienceschemistry.chemical_compoundConfocal laser scanning microscopyFluorescence Resonance Energy TransferOrganic chemistryAnimalsCyanineMolecular BiologyFluorescent DyesMicroscopy ConfocalOrganic ChemistryfungiCarbocyaninesCopperMitochondriaRats030104 developmental biologyFörster resonance energy transferchemistryMitochondrial targetingClick chemistryBiophysicsMolecular MedicineClick ChemistryCopperConjugatedescription
Cyanine (Cy) dyes show a general propensity to localize in polarized mitochondria. This mitochondriotropism was used to perform a copper-free click reaction in the mitochondria of living cells. The in organello reaction of dyes Cy3 and Cy5 led to a product that was easily traceable by Forster resonance energy transfer (FRET). As determined by confocal laser scanning microscopy, the Cy3-Cy5 conjugate showed enhanced retention in mitochondria, relative to that of the starting compounds. This enhancement of a favorable property can be achieved by synthesis in organello, but not outside mitochondria.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-07-31 | ChemBioChem |