Rapid and simultaneous high-performance liquid chromatography assay of polyamines and monoacetylpolyamines in biological specimens

6533b7dafe1ef96bd126ed10

RESEARCH PRODUCT

Rapid and simultaneous high-performance liquid chromatography assay of polyamines and monoacetylpolyamines in biological specimens

Maria Concetta Gueli Maria Rita Schiavo Concetta M.a. Nicotra P. C. Rindina Gennaro Taibi R. Muratore

subject

Chromatography medicine.diagnostic_test organic chemicals Biogenic Polyamines Reproducibility of Results General Chemistry Reversed-phase chromatography Reference Standards Sensitivity and Specificity High-performance liquid chromatography Benzoic anhydride chemistry.chemical_compound Benzoyl chloride chemistry Spectrophotometry medicine Spectrophotometry Ultraviolet Derivatization Quantitative analysis (chemistry)Chromatography High Pressure Liquid Benzoic acid

description

A rapid, resolutive and reproducible reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for polyamines and acetylpolyamines by adopting pre-column derivatization with benzoyl chloride. In a single run lasting less than 15 min ten polyamines were separated as well as traces of benzoic acid, methylbenzoate and benzoic anhydride. These contaminants, produced during the derivatization reaction, were almost all eliminated by washing steps envisaged in the same procedure. This simple and sensitive method can be applied to routine determination of polyamines in biological samples. A fine application of this procedure to the determination of endogenous content of polyamines in chick embryo retina was reported.

year	journal	country	edition	language
2000-10-24	Journal of Chromatography B: Biomedical Sciences and Applications

https://doi.org/10.1016/s0378-4347(00)00314-5

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