6533b7dafe1ef96bd126ed3c

RESEARCH PRODUCT

Isolation and Culture of Oligodendrocytes

Wen Ping Kuo-elsnerChaitali MukherjeeChristina MüllerMikael SimonsMarie-theres WeilIsabelle SchäferGiselheid Schulz-ëberlin

subject

methods [Cell Separation]0303 health sciencesMagnetic-activated cell sortingMagnetic PhenomenaOligodendrocyte differentiationOligodendrocyte progenitorCell DifferentiationBiologyFlow CytometryIsolation (microbiology)Coculture TechniquesCell biologyMice03 medical and health sciences0302 clinical medicineAnimals Newbornddc:570cytology [Neurons]methods [Cell Culture Techniques]Animalscytology [Oligodendroglia]Cells Cultured030217 neurology & neurosurgeryCell Proliferation030304 developmental biology

description

Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.

yearjournalcountryeditionlanguage
2019-01-01
https://doi.org/10.1007/978-1-4939-9072-6_5