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RESEARCH PRODUCT

In Vitro Interactions of C-ANCA (Antibodies to Proteinase 3) with Human Endothelial Cells

Elena CsernokElisabeth HermannWolfgang L. GrossWerner-j. MayetKarl-h. Meyer Zum Büschenfelde

subject

C-ANCAmedicine.diagnostic_testmedicine.drug_classBiologyMonoclonal antibodyMolecular biologyIn vitroWestern blotProteinase 3Myeloblastinmedicinebiology.proteincardiovascular diseasesAntibodyAnti-neutrophil cytoplasmic antibody

description

Several concepts concerning the pathogenicity of antineutrophil cytoplasmic antibodies (ANCA) exist, but till now only sparse data about ANCA-endothelial interactions are available. In this study we have investigated the expression of proteinase 3 (PR-3) in human umbilical endothelial cells (HEC) using purified anti-PR3 antibodies (C-ANCA) of patients with Wegener’s granulomatosis (WG) and monoclonal antibodies to PR-3 (human and murine) as probes. Performing cytoELISAs, laser scanning microscopy and Western blot we were able to show that treatment of HEC with IL-1-alpha led to an increased PR-3 expression in the cytoplasm and to a transient translocation into the EC-membrane. Representing an important missing link of ANCA-endothelial interactions, our data give a hint at a possible direct pathogenicity of anti-PR-3 antibodies in WG and other vasculitides.

yearjournalcountryeditionlanguage
1993-01-01
https://doi.org/10.1007/978-1-4757-9182-2_18