6533b7dafe1ef96bd126f58c

RESEARCH PRODUCT

Generation of monoclonal antibodies against human regulatory T cells.

Wolfgang RistHelmut JonuleitMartin LenterFranz-joseph SchneiderChristian BeckerHeinz Hoschützky

subject

Anticorps monoclonalmedicine.drug_classImmunologyReceptors Antigen T-Cellchemical and pharmacologic phenomenaCell SeparationBiologyMonoclonal antibodyT-Lymphocytes RegulatoryFlow cytometryEpitopesMiceImmune systemAntibody SpecificitymedicineImmunology and AllergyAnimalsHumansIL-2 receptorLeukapheresisImmunization ScheduleHybridomasmedicine.diagnostic_testInterleukin-2 Receptor alpha SubunitFOXP3Antibodies Monoclonalhemic and immune systemsForkhead Transcription FactorsT lymphocyteFlow CytometryImmunizationImmunologyFemaleEpitope Mapping

description

Abstract Natural CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers and to identify targets for a therapeutic modulation of Tregs. Here we present a methodology optimized to efficiently induce and select mAb against human Tregs by repeated immunization of mice with Tregs from a single donor and a differential two-step flow cytometry-based hybridoma screening procedure.

yearjournalcountryeditionlanguage
2009-08-28Journal of immunological methods
10.1016/j.jim.2010.01.002https://pubmed.ncbi.nlm.nih.gov/20096700