6533b7dbfe1ef96bd126ff66
RESEARCH PRODUCT
Improvement of the butyl methacrylate-paraffin embedment.
Arthur DalgleishJames McmillanPaul C. EngenPaul J. Mcmillansubject
Materials scienceStaining and LabelingEmbedmentHistological Techniquestechnology industry and agriculturechemistry.chemical_elementBenzoyl peroxideNitrogenButyl methacrylateVacuum furnaceFixativeschemistryChemical engineeringPolymerizationPolymethacrylic AcidsParaffinTorrPolymer chemistryMicroscopymedicineMethacrylatesAnatomymedicine.drugdescription
The excellent butyl methacrylate-paraffin method as an embedment for light microscopy has been technically improved. More uniform and reproducible polymerization has been obtained by using a vacuum oven to degas the polymerizing mixture and to replace the air with nitrogen at 650 Torr. The amount of benzoyl peroxide required must be determined for each batch of butyl methacrylate. A teflon lined, reusable metal mold and a method of one-step blocking of tissues in preparation for sectioning are also described.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1983-05-01 | Stain technology |