6533b7dbfe1ef96bd12701d2

RESEARCH PRODUCT

Ferrocenyl glycopeptides as electrochemical probes to detect autoantibodies in multiple sclerosis patients' será

Feliciana Real-fernándezFrancesca NutiJérôme BayardonChristophe DarcelAnna Maria PapiniRita Meunier-prestAmélie ColsonSylvain JugéMario ChelliElisa PeroniFrancesco Lolli

subject

Multiple SclerosisMetallocenesCarboxylic acidBiophysics010402 general chemistry01 natural sciencesBiochemistryBiomaterialsImmunoenzyme Techniques[ CHIM.CATA ] Chemical Sciences/CatalysismedicineElectroanalytical methodElectrochemistryMoietyHumansFerrous CompoundsAntigensComputingMilieux_MISCELLANEOUSAutoantibodieschemistry.chemical_classification010405 organic chemistryChemistryMultiple sclerosisOrganic ChemistryAutoantibodyGlycopeptidesGeneral Medicine[CHIM.CATA]Chemical Sciences/Catalysismedicine.diseaseGlycopeptide0104 chemical sciences3. Good healthAmino acidSolutionsBiochemistryMolecular ProbesGoldCyclic voltammetryChromatography Liquid

description

Abstract Glycopeptide analogues of CSF114(Glc), modified at N-terminus with new ferrocenyl carboxylic acid and a new ferrocenyl-thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these "electrochemical probes" did not affect autoantibody recognition both in SP-ELISA and in inhibition experiments. By electrochemical monitoring the interactions of the modified peptides Fc-CSF114(Glc) and 4-FcPhP(S)Abu-CSF114(Glc) with the autoantibodies, we demonstrated that autoantibodies could be detected with a sensitivity comparable to ELISA method. The new electrochemical probes can be proposed to characterize autoantibodies as biomarkers of multiple sclerosis by a simple, rapid, and reproducible cyclic voltammetry-based diagnostic methodology.

yearjournalcountryeditionlanguage
2008-02-15
https://hal.archives-ouvertes.fr/hal-00342823