6533b7dbfe1ef96bd127024d

RESEARCH PRODUCT

Cow's milk–protein allergy as a cause of anal fistula and fissures: A case report

subject

description

The amplified ELISA assay results correlate well with the standard ELISA results reported previously from our laboratory. Previously it has been necessary to use high flow samplers or long sampling times to measure airborne Fel d 1 concentrations in the range reported here.5 The amplified assay might allow measurements with very short sampling periods to better describe dose-response relationships, and may also be applied to measure airborne concentrations of allergens such as cockroach or dust mite that are airborne at very low concentrations. Our finding that Fel d 1 allergen is measurable in all samples from homes without cats confirms the ubiquitous nature of cat allergen and may help to explain the high sensitization rate among people who do not live with cats.6 The poor correlation between air and dust samples supports the finding by others7 that settled dust levels may not provide an accurate estimate of airborne exposure. The practical importance of this more sensitive assay is that we can now measure airborne Fel d 1 concentrations with sampling techniques that involve lower flows, portable equipment, and shorter sampling times. Although the clinical relevance of low airborne Fel d 1 levels is yet to be determined, the amplified assay provides a useful technique to examine the question experimentally.