6533b7dbfe1ef96bd127028d

RESEARCH PRODUCT

Pulsed-field gel electrophoresis for the discrimination of Oenococcus oeni isolates from different wine-growing regions in Germany

Melanie LarisikaHelmut KönigHarald Claus

subject

DNA BacterialGel electrophoresisWineStrain (biology)WineHigh capacityGeneral MedicineBiologybiology.organism_classificationMicrobiologyElectrophoresis Gel Pulsed-FieldMicrobiologyGram-Positive CocciRestriction enzymegenomic DNASpecies SpecificityGermanyFermentationPulsed-field gel electrophoresisCluster AnalysisFood scienceDeoxyribonucleases Type II Site-SpecificPhylogenyFood ScienceOenococcus oeni

description

Reliable techniques are needed for the identification individual Oenococcus oeni strains with desirable flavor characteristics and to monitor the survival and contribution of inoculated and indigenous bacteria. Therefore, we investigated the suitability of pulsed-field gel electrophoresis (PFGE) for the discrimination of 65 O. oeni isolates from six different wine-producing regions in Germany. Among the restriction enzymes tested, genomic DNA digestions with Sfi I were most effective by displaying 56 (86%) different banding profiles. Our results underline the high capacity of PFGE for strain identification and differentiation. Cluster analysis of the DNA restriction patterns revealed no distinct region specificity of O. oeni populations.

yearjournalcountryeditionlanguage
2008-03-01International Journal of Food Microbiology
https://doi.org/10.1016/j.ijfoodmicro.2007.11.081