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RESEARCH PRODUCT
Analysis of Invariant Chain Processing in 3 Day Cultured Rat Langerhans Cells
Ursula NeißKonrad Reskesubject
chemistry.chemical_compoundMHC class IIbiologyBiosynthesischemistryExogenous antigenDownregulation and upregulationbiology.proteinPeptide bindingMajor histocompatibility complexIn vitroCell biologyInvariant chaindescription
MHC class II molecules, critical peptide binding elements involved in the presentation of exogenous antigen to T helper cells, are expressed constitutively by Langerhans cells (LC) within their epidermal microenvironment. Several studies in mouse and man demonstrated, that short term in vitro culture of LC entails remarkable functional and penotypic alterations, including a profound increase of class II elements exposed at the LC’s surface1. Biosynthetic analysis revealed a downregulation of class II synthesis during the culture period2,3. In recent work on rat LC we described the uncoupling of the coordinately regulated biosynthesis of class II and invariant chain proteins in the course of culture4. Thus, LC undergoing 3 days of in vitro culture synthesize very high levels of γ-chains in the almost complete absence of class II synthesis. We extended these studies to explore invariant chain synthesis and processing in 3 day cultured LC more precisely.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-01-01 |