6533b7dbfe1ef96bd12714b1
RESEARCH PRODUCT
Isolation, culture and plant regeneration from protoplasts of Sideritis angustifolia
Juan SeguraJoao Luiz Carvalho FariaIgor Kostenyuksubject
Plating efficiencyPhysiologyRegeneration (biology)fungifood and beveragesPlant ScienceProtoplastBiologybiology.organism_classificationMurashige and Skoog mediumCell cultureCallusBotanyShootSideritisAgronomy and Crop Sciencedescription
Summary Factors promoting high yields of viable protoplasts from leaves of Sideritis angustifolia are reported. Isolated protoplasts divided and gave rise to callus when plated in modified liquid MS medium with growth regulators. Best plating efficiency was obtained when protoplast were cultured in the presence of 6 μM BA and 2 μM NAA. Calli were then transferred onto solidified regeneration media. Adventitious bud differentiation was only observed in calli grown on medium containing 8 μM BA and 2 μM NAA. These buds proliferated quickly when placed on hormone-free medium and subsequendy regenerated new buds and shoots. Once developed shoots were excised, the remaining tissues maintained this regenerative capability in successive subcultures. Regenerated shoots were easily rooted on MS medium without growth regulators.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-01-01 |