6533b7dbfe1ef96bd1271590
RESEARCH PRODUCT
Biphenyl and fluorinated derivatives: liver enzyme-mediated mutagenicity detected in Salmonella typhimurium and Chinese hamster V79 cells.
Hansruedi GlattElke AnklamLarry W. Robertsonsubject
MaleSalmonella typhimuriumendocrine systemChinese hamsterAmes testCell LineToxicologychemistry.chemical_compoundStructure-Activity RelationshipCricetulusCricetinaeAnimalsBiotransformationBiphenylbiologyChemistryMutagenicity TestsBiphenyl CompoundsRats Inbred StrainsGeneral MedicineMetabolismbiology.organism_classificationEnterobacteriaceaeIn vitroRatsBiochemistryMicrosomal epoxide hydrolaseMicrosomes LiverPolybrominated Biphenylsdescription
Abstract Hepatocarcinogenic polychlorinated and polybrominated biphenyls usually show negative results in in vitro mutagenicity assays. Problems in their testing result from their low water solubility and their slow rate of metabolism. We therefore investigated better soluble model compounds, namely biphenyl and its 3 possible monofiuorinated derivatives. In the direct test, these compounds proved tobe nonmutagenic in Salmonella typhimurium TA98 and TA100 (reversion to histidine prototrophy) and in Chinese hamster V79 cells (acquisition of resistance to 6-thioguanine). However, when the exposure was carried out in the presence of NADPH-fortified postmitochondrial fraction of liver homogenate from Aroclor 1254-treated rats. all 4 compounds showed mutagenic activity in V79 cells. 3-Fluorobiphenyl produced strong mutagenic effects in S. typhimurium TA100 as well, whereas the other biphenyls were inactive. In strain TA9J8, 3- and 4-fluorobiphenyl showed mutagenic activity. This mutagenicity was enhanced in the presence of 1,1,1-trichloropropene 2,3-oxide, an inhibitor of microsomal epoxide hydrolase, thus suggesting that epoxides may be active metabolites.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-03-01 | Mutation research |