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RESEARCH PRODUCT
Colorado potato beetle chymotrypsin genes are differentially regulated in larval midgut in response to the plant defense inducer hexanoic acid or the Bacillus thuringiensis Cry3Aa toxin
Sara S. OltraVictor M. Ruiz-arroyoMarko PetekMaría José López-galianoCarolina RausellInmaculada García-roblesM. Dolores Realsubject
0106 biological sciences0301 basic medicineGenes Insectmedicine.disease_cause01 natural sciencesMicrobiologyHemolysin Proteins03 medical and health scienceschemistry.chemical_compoundBacterial ProteinsBacillus thuringiensisPlant defense against herbivorymedicineAnimalsChymotrypsinCaproatesEcology Evolution Behavior and SystematicsSolanum tuberosumHexanoic acidChymotrypsinBacillus thuringiensis ToxinsbiologyToxinfungiColorado potato beetlefood and beveragesMidgutbiology.organism_classificationEnzyme assayColeopteraEndotoxins010602 entomology030104 developmental biologyGene Expression RegulationchemistryLarvabiology.proteinDigestive Systemdescription
When Colorado potato beetle larvae ingested potato plants treated with the plant defense inducer compound hexanoic acid, midgut chymotrypsin enzyme activity increased, and the corresponding chymotrypsin genes were differentially expressed, evidence of the larval digestive proteolytic system's plasticity. We previously reported increased susceptibility to Cry3Aa toxin in larvae fed hexanoic acid treated plants. Here we show that the most expressed chymotrypsin gene in larvae fed hexanoic acid treated plants, CTR6, was dramatically downregulated in Cry3Aa intoxicated larvae. lde-miR-965-5p and lde-miR-9a-5p microRNAs, predicted to target CTR6, might be involved in regulating the response to hexanoic acid but not to Cry3Aa toxin.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-01-03 | Journal of Invertebrate Pathology |