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RESEARCH PRODUCT
On the Relationship between Jetted Inks and Printed Biopatterns:Molecular-Thin Functional Microarrays of Glucose Oxidase
Giuseppe CompagniniGiuseppe ArrabitoSebania LibertinoV. AielloBruno PignataroChiara Musumecisubject
GlycerolSiliconSurface PropertiesProtein Array Analysischemistry.chemical_elementNanotechnologyASPERGILLUS-NIGERMicrometreSIO2 SURFACESGlucose Oxidase inkjet printingGlucose OxidaseStructure-Activity RelationshipElectrochemistryGeneral Materials ScienceGlucose oxidaseSilicon oxideSpectroscopybiologyInkwellPulse (signal processing)ChemistryPulse durationSurfaces and InterfacesCondensed Matter PhysicsPiezoelectricityChemical engineeringIMMOBILIZATIONbiology.proteinMicroscopy Electron ScanningInkHIGH HYDROSTATIC-PRESSUREdescription
Arrays of circular spots of glucose oxidase have been obtained on functionalized silicon oxide by piezoelectric inkjet printing and the enzymatic activity toward glucose recognition has been monitored. The addition of glycerol to the molecular ink allows to obtain high spot definition and resolution (tens of micrometers wide; one molecule tall), but in spite of its well-known structural stabilizing properties, in dynamic conditions it may lead to increased protein stresses. The jetting voltage and pulse length have been found to be critical factors for both activity retention and pattern definition. High voltages and pulse lengths results in stress effects along with the loss of activity, which, at least in our experimental conditions, has been found to be recovered in time.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-01-01 |