6533b7dcfe1ef96bd127208a
RESEARCH PRODUCT
In Vitro Cultured Islet‐Derived Progenitor Cells of Human Origin Express Human Albumin in Severe Combined Immunodeficiency Mouse Liver In Vivo
Henryk ZulewskiDirk PrawittBernhard ZabelAndreas ReichenbachWiebke SchormannMatthias HermesMichael EberhardtBeat MüllerMarc-alexander Von MachMarc BrulportL. S. WeilemannJan G. HengstlerJens Groschesubject
Time FactorsCell TransplantationTransplantation HeterologousMice SCIDBiologyIslets of LangerhansMiceIn vivoAlbuminsmedicineAnimalsHumansRNA MessengerOrganic ChemicalsProgenitor cellCells CulturedFluorescent DyesSevere combined immunodeficiencygeographygeography.geographical_feature_categoryReverse Transcriptase Polymerase Chain ReactionStem CellsTransdifferentiationAlbuminCell DifferentiationCell Biologymedicine.diseaseIsletImmunohistochemistryMolecular biologyIn vitroChromosome BandingPhenotypeLiverMicroscopy FluorescenceKaryotypingImmunologyMolecular MedicineStem cellDevelopmental Biologydescription
Studies in rodents suggest the presence of a hepatopancreatic stem cell in adult pancreas that may give rise to liver cells in vivo. The aim of the present study was to determine the ability of human islet-derived cells to adopt a hepatic phenotype in vivo. Cultured human islet-derived progenitor cells that did not express albumin in vitro were stained with the red fluorescent dye PKH26 and injected into the liver of severe combined immunodeficiency mice. After 3 or 12 weeks, red fluorescent cells were detected in 11 of 15 livers and were mostly single cells that were well integrated into the liver tissue. Human albumin was found in 8 of 11 animals by immunohistochemistry, and human albumin mRNA was detected in 4 of 10 host livers. The mechanism underlying this phenomenon seems to be transdifferentiation, because human and mouse albumin were found to be expressed in distinct cells in the host liver.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-12-08 | STEM CELLS |